Description: The PARPtrap™ Combo Assay Kit for PARP1 and PARP2 is a kit designed to measure DNA complex formations of PARP1 and PARP2 enzymes in a high throughput screening assay using fluorescence polarization (FP). The key to the PARPtrap™ Combo Assay Kit are the fluorescent-labeled DNA probes recognized by PARP1 and PARP2. In the absence of ribosylation, PARP1/2 binds to the DNA fluorescent probe, forming a large complex and resulting in the emission of highly polarized light. However, after autoribosylation, PARP1/2 dissociates from the oligonucleotide duplex, which then rotates freely, emitting less polarized light (Fig. 1). Addition of a PARP inhibitor results in trapping of PARP1/2 to the fluorescent-labeled DNA, and increases the FP signal in a dose dependent manner.The combo kit is particularly useful to directly and specifically compare, in a single assay, the effect and potency of a compound on PARP1 and PARP2. The PARPtrap™ Combo Assay Kit for PARP1 and PARP2 is a homogeneous fluorescence polarization assay. The FP signal is measured using a fluorescent microplate reader capable of measuring fluorescence polarization.

Description: MTT Cell Proliferation and Viability Assay Kit

Description: This product includes 40 µl of 100 x Reagent A, 40 µl of 100 x Reagent B, 200 µl of 100 x fluorescence dye and 50 µl of 10 mM potassium iodide solution.

Description: This product includes 100 ul of 1000 x MCA dye and 30 ul of 1 mM CuCl2. It is for 1000 assays using 384-well plates (30 ul of sample volume) or 300 assays using 96-well plates (100 l of sample volume). Cuvettes may also be used for measurements. The kit also provides a 1 mM CuCl2 solution for standard curve.

Description: This product includes 40 ml of Reagent BP. It is for assays using 96-well plates. Cuvettes may also be used for measurements.

Description: This product includes 400 ul of 100 x Dye R53. It is for measurement of 200 samples using 96-well plates. Cuvettes or 383-well plates may also be used for measurements.

Description: This product includes 1 ml of Reagent CLA and 0.1 ml of 10 mM NaCl solution. It is for measurement of 100 samples using 96-well plates. Cuvettes may also be used for measurements.

Description: This product includes 6 ml of Reagent PMX1 and 6 ml of Reagent PMX2. It is for 200 assays using 96-well plates. Cuvettes may also be used for the assay.

Description: This product includes 4 ml of Reagent A, 0.080 ml of 50 x Reagent B, and 2 ml of 10 x Reagent C. It is for 200 assays using 96-well plates. Cuvettes may also be used for measurements.

Description: This product includes 750 ul 10 x C53 Dye and 50 ul of 10 mM Histidine. It is for measurement of 200 samples using 96-well plates. Cuvettes may also be used for measurements.

Description: This product includes 0.5 ml of 10 x VAN dye, 5.5 ml of Assay buffer, 5 ml of reagent A. It is for measurement of 100 samples using 96-well plates.

Description: Although JC-1 is widely used in many labs, its poor water solubility makes it hard to use for some applications.

Description: This product includes 1 ml of 10 x C33 dye for 200 assays using 96-well plates.