Development of a highly effective low-cost vaporized hydrogen peroxide-based method for disinfection of personal protective equipment for their selective reuse during pandemics
Background: Personal Protective Equipment (PPE) is required to soundly work with organic brokers of bacterial (i.e. Mycobacterium tuberculosis) or viral origin (Ebola and SARS). COVID-19 pandemic particularly has created unexpected public well being challenges together with a international scarcity of PPE wanted for the protection of well being care employees (HCWs). Although ample shares of PPE are presently out there, their crucial scarcity could develop quickly because of enhance in demand and depletion of current provide traces. To empower our HCWs and guarantee their continued safety, proactive measures are urgently required to develop procedures to soundly decontaminate the PPEs to permit their “selective reuse” during contingency conditions.
Methods: Herein, now we have efficiently developed a decontamination method primarily based on vaporized hydrogen peroxide (VHP). We have used a vary of focus of hydrogen peroxide to disinfect PPE (coveralls, face-shields, and N-95 masks). To guarantee a correct disinfection, now we have evaluated three organic indicators specifically Escherichia coli, Mycobacterium smegmatis and spores of Bacillus stearothermophilus, thought-about because the gold customary for disinfection processes. We subsequent evaluated the impression of repeated VHP therapy on bodily options, permeability, and material integrity of coveralls and N-95 masks. Next, we carried out Scanning Electron Microscopy (SEM) to judge microscopic adjustments in fiber thickness of N-95 masks, soften blown layer or coverall physique fits. Considering the truth that any disinfection process ought to be capable to meet native necessities, our research included varied regionally procured N-95 masks and coveralls out there at our institute All India Institute of Medical Sciences (AIIMS), New Delhi, India. Lastly, the sensible utility of VHP method developed herein was ascertained by operationalizing a devoted analysis facility disinfecting used PPE during COVID-19.
Results: Our prototype research present that a single VHP cycle (7-8% Hydrogen peroxide) may disinfect PPE and PPE housing room of about 1200 cubic ft (size10 ft × breadth 10 ft × peak 12 ft) in lower than 10 min, as famous by a full loss of B. stearothermophilus spore revival. The outcomes are constant and reproducible as examined in over 10 cycles in our settings. Further, repeated VHP therapy didn’t lead to any bodily tear, deformity or different considerable change within the coverall and N-95 masks. Our permeation assessments evaluating droplet penetration didn’t reveal any change in permeability post-VHP remedies.
Development of a highly effective low-cost vaporized hydrogen peroxide-based method for disinfection of personal protective equipment for their selective reuse during pandemics
Also, SEM evaluation certainly revealed no vital change in fiber thickness or harm to fibers of coveralls or soften blown layer of N-95 masks important for filtration. There was no change in consumer consolation and expertise following VHP therapy of PPE. Based on outcomes of these research, and parameters developed and optimized, an institutional analysis facility to disinfect COVID-19 PPE is efficiently established and operationalized with greater than 80% restoration price for used PPE post-disinfection.
Conclusions: Our research, subsequently, efficiently establishes the utility of VHP to successfully disinfect PPE for a attainable reuse as per the necessities. VHP therapy didn’t harm coveralls, trigger bodily deformity and likewise didn’t alter material structure of soften blown layer.
Description: Quantitativesandwich ELISA kit for measuring Human vascular endothelial cell growth factor receptor 1 (VEGFR-1/Flt1) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Human vascular endothelial cell growth factor receptor 1 (VEGFR-1/Flt1) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Human Vascuoar endothelial cell growth factor receptor 1(VEGFR-1/Flt1)ELISA Kit
Description: Quantitativesandwich ELISA kit for measuring Rat Vascular endothelial cell growth factor receptor 1, VEGFR-1/Flt1 in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Rat Vascular endothelial cell growth factor receptor 1, VEGFR-1/Flt1 in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Rat Vascuoar endothelial cell growth factor receptor 1(VEGFR-1/Flt1)ELISA Kit
Description: Quantitativesandwich ELISA kit for measuring Mouse Vascular endothelial cell growth factor receptor 1, VEGFR-1/Flt1 in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.
Description: Quantitativesandwich ELISA kit for measuring Mouse Vascular endothelial cell growth factor receptor 1, VEGFR-1/Flt1 in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.
Description: Vascular endothelial growth factor receptor 1 (FLT1) is a protein that in humans is encoded by the FLT1 gene. Oncogene FLT belongs to the src gene family. It is mapped to 13q12. The deduced 1,338-amino acid protein has a calculated molecular mass of 150.6 kD. It has a 758-amino acid extracellular domain, followed by a 22-amino acid transmembrane region and a 558-amino acid cytoplasmic region containing a cluster of basic amino acids and a tyrosine kinase domain. sFLT-1 was identified in placenta, adult lung, kidney, liver and uterus. Like other members of this family, it shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation.
Description: VEGFR1 is high affinity receptors for vascular endothelial growth factors (VEGFs). On the basis of structural similarity to VEGFR1 and 2, it has been speculated that Flt-4 might represent a third receptor for either VEGF or a VEGF-related ligand.
Description: VEGFR1 is high affinity receptors for vascular endothelial growth factors (VEGFs). On the basis of structural similarity to VEGFR1 and 2, it has been speculated that Flt-4 might represent a third receptor for either VEGF or a VEGF-related ligand.
Description: VEGFR1 is high affinity receptors for vascular endothelial growth factors (VEGFs). On the basis of structural similarity to VEGFR1 and 2, it has been speculated that Flt-4 might represent a third receptor for either VEGF or a VEGF-related ligand.
Description: VEGFR1 is high affinity receptors for vascular endothelial growth factors (VEGFs). On the basis of structural similarity to VEGFR1 and 2, it has been speculated that Flt-4 might represent a third receptor for either VEGF or a VEGF-related ligand.
Description: VEGFR1 is high affinity receptors for vascular endothelial growth factors (VEGFs). On the basis of structural similarity to VEGFR1 and 2, it has been speculated that Flt-4 might represent a third receptor for either VEGF or a VEGF-related ligand.
Description: VEGFR1 is high affinity receptors for vascular endothelial growth factors (VEGFs). On the basis of structural similarity to VEGFR1 and 2, it has been speculated that Flt-4 might represent a third receptor for either VEGF or a VEGF-related ligand.
Description: A sandwich ELISA kit for quantitative measurement of Human VEGFR1/FLT1 (Vascular Endothelial Growth Factor Receptor 1) in samples from Serum, Plasma, Cell supernatant
Description: Enzyme-linked immunosorbent assay kit for quantification of Mouse FLT1/VEGFR1 in samples from serum, plasma, tissue homogenates and other biological fluids.
Description: A Monoclonal antibody against Human VEGF-R1 / FLT-1 - With BSA and Azide. The antibodies are raised in Mouse and are from clone FLT1/659. This antibody is applicable in E
Monoclonal VEGF-R1 / FLT-1 Antibody - With BSA and Azide, Clone: FLT1/658
Description: A Monoclonal antibody against Human VEGF-R1 / FLT-1 - With BSA and Azide. The antibodies are raised in Mouse and are from clone FLT1/658. This antibody is applicable in E
Monoclonal VEGF-R1 / FLT-1 Antibody - Without BSA and Azide, Clone: FLT1/659
Description: A Monoclonal antibody against Human VEGF-R1 / FLT-1 - Without BSA and Azide. The antibodies are raised in Mouse and are from clone FLT1/659. This antibody is applicable in IF, FC
Monoclonal VEGF-R1 / FLT-1 Antibody - Without BSA and Azide, Clone: FLT1/658
Description: A Monoclonal antibody against Human VEGF-R1 / FLT-1 - Without BSA and Azide. The antibodies are raised in Mouse and are from clone FLT1/658. This antibody is applicable in IF, FC
Description: For quantitative detection of mouse VEGFR1 in cell culture supernates and serum.
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We noticed that disinfection course of was profitable persistently and subsequently consider that the VHP-based decontamination mannequin may have a common applicability and utility. This course of could be simply and economically scaled up and could be instrumental in easing international PPE shortages in any biosafety facility or in well being care settings during pandemic scenario equivalent to COVID-19.
Kirsten
